testis-specific genes annotated  (Human Protein Atlas)

Journal: Clinical Epigenetics

Article Title: Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

doi: 10.1186/1868-7083-4-23

Figure Lengend Snippet: Representative electrophoretogram of rat testis showing bands of histones, ubiqutin, cremΔC-G genes implicated in sperm chromatin remodelling. ( A ) th3, h3s, th2a, th2b. ( B ) h2as. ( C ) h2bs. ( D ) ubB (Ubiquitin). ( E ) h4, cremΔC-G . Lane M: NEB 100 bp Ladder. Lane C: Control samples. Lane F: FD-treated samples. Lane P: CPA-treated samples. NS: non-specific bands.

Article Snippet: The conditions of PCR used to amplify the genes for 35 cycles were: denaturation at 94°C × 2 min and 94°C × 30 s; annealing at 55°C × 1 min; extension for 68°C × 1 min; final extension at 68°C × 7 min. Gene specific primers for ube1 , ube2d2 , ure b1 / e3 , cdyl , brdt , hdac1 , hdac6 , miwi (RNA-binding protein in chromatoid body transports ACT (activator of cyclic AMP response element modulator-CREMτ), mbd2 (methyl CpG binding protein), pem , h2as (somatic), h2bs (somatic), h3s (somatic), th2a (testis-specific), th2b (testis-specific), th3 (testis-specific) were used for the amplification by biplex PCR as previously described (Invitrogen primer details in Table ) [ ].

Techniques:

Journal: Clinical Epigenetics

Article Title: Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

doi: 10.1186/1868-7083-4-23

Figure Lengend Snippet: Expression of chromatin condensing genes in FD/CPA-treated rat testis

Article Snippet: The conditions of PCR used to amplify the genes for 35 cycles were: denaturation at 94°C × 2 min and 94°C × 30 s; annealing at 55°C × 1 min; extension for 68°C × 1 min; final extension at 68°C × 7 min. Gene specific primers for ube1 , ube2d2 , ure b1 / e3 , cdyl , brdt , hdac1 , hdac6 , miwi (RNA-binding protein in chromatoid body transports ACT (activator of cyclic AMP response element modulator-CREMτ), mbd2 (methyl CpG binding protein), pem , h2as (somatic), h2bs (somatic), h3s (somatic), th2a (testis-specific), th2b (testis-specific), th3 (testis-specific) were used for the amplification by biplex PCR as previously described (Invitrogen primer details in Table ) [ ].

Techniques: Expressing

Journal: Clinical Epigenetics

Article Title: Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

doi: 10.1186/1868-7083-4-23

Figure Lengend Snippet: Details of PCR primers used for molecular genetic studies

Article Snippet: The conditions of PCR used to amplify the genes for 35 cycles were: denaturation at 94°C × 2 min and 94°C × 30 s; annealing at 55°C × 1 min; extension for 68°C × 1 min; final extension at 68°C × 7 min. Gene specific primers for ube1 , ube2d2 , ure b1 / e3 , cdyl , brdt , hdac1 , hdac6 , miwi (RNA-binding protein in chromatoid body transports ACT (activator of cyclic AMP response element modulator-CREMτ), mbd2 (methyl CpG binding protein), pem , h2as (somatic), h2bs (somatic), h3s (somatic), th2a (testis-specific), th2b (testis-specific), th3 (testis-specific) were used for the amplification by biplex PCR as previously described (Invitrogen primer details in Table ) [ ].

Techniques: Variant Assay